Insertion junction: CLIP2.021156_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.021156
Chromosome:chromosome 3
Location:6522843
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g194050 (1 of 274) IPR020683 - Ankyrin repeat-containing domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGGGATCTGCGGAATAAAATACAACGAGGGAGCGGTCACACGCAAAGG
Internal bar code:AATCCGCATCATATAGATCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1491
LEAP-Seq percent confirming:76.4706
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:TCCATACATGATCGCCTGCC
Suggested primer 2:GAAGCTGCGAGTCGTATCGA