| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.021160 |
| Chromosome: | chromosome 10 |
| Location: | 2875787 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439600 | (1 of 1) PTHR11649//PTHR11649:SF62 - MSS1/TRME-RELATED GTP-BINDING PROTEIN // PROTEIN SEY1 HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTAAACCCCCGTCTACCCTCCACCACTTCCTTAACTAATCATGAATGT |
| Internal bar code: | GACTATCTAATTGCGGCTTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2348 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCCATGGTCTACGGTATC |
| Suggested primer 2: | TTGTTTGAGCTGTGTTCGCG |