Insertion junction: CLIP2.021180_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.021180
Chromosome:chromosome 8
Location:3432465
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g378300 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAAACCAGGTCAGCTGCGTTTGTTGGAGGCGTGATGAGGAGGGGTGAT
Internal bar code:TACGTTGTAGTGCTTGGGCGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:4836
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:146
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:146

Suggested primers for confirmation by PCR

Suggested primer 1:GACAACACACAGTCACGCAC
Suggested primer 2:CACCAATACTCACCCACCCC