| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.021181 |
| Chromosome: | chromosome 3 |
| Location: | 3726427 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169050 | (1 of 1) PTHR12183//PTHR12183:SF18 - UNCHARACTERIZED RING ZINC FINGER-CONTAINING PROTEIN // RING-TYPE UBIQUITIN E3 LIGASE | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTTGACGCCCGCCGCCGCCGCGCGCTCCGCCGCCCGTTGGCGCCGCAC |
| Internal bar code: | TTCGTGACTCTTTATAGGAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1697 |
| LEAP-Seq percent confirming: | 97.9592 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTTCGTCCTCGACATCC |
| Suggested primer 2: | CGCCGCTTGCACTTCTTATC |