Insertion junction: CLIP2.021188_4

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.021188
Chromosome:chromosome 16
Location:135956
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCTGCCGCGAAGGAGGCTGCCATAATCCCCAACTCCGCACAATGTACA
Internal bar code:CAATCCTATAAAGTGCGTGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:641
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGCGCTTTTTCGTGTTGC
Suggested primer 2:GAGCCAGGTTATCTCTCCGC