| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.021189 |
| Chromosome: | chromosome 5 |
| Location: | 2153528 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g237100 | MOT52 | Predicted protein; (1 of 1) K16535 - lisH domain-containing protein FOPNL (FOPNL, FOR20) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCAAGAGCGCACTCACAGCTGGGCGGAGTTGGGCCCCTCAGGCACGT |
| Internal bar code: | GTTGGTTACCAGCGCATTTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2152 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACCGTGTCTGTGTTCCTA |
| Suggested primer 2: | CTGAGTCCTGACCACCACAC |