| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.021190 |
| Chromosome: | chromosome 17 |
| Location: | 3287633 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722100 | SSA3 | cilia-sensing, structure and/or assembly; (1 of 1) PTHR12771:SF2 - ELMO DOMAIN-CONTAINING PROTEIN 3 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGCTCCGGCGGCGCCAGGCACAGCAGCCGCGCGCAGAGGCCGGGAGGC |
| Internal bar code: | AACCAGGGTCGGTACACGAACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2112 |
| LEAP-Seq percent confirming: | 98.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTTGCAAAGGCCTTTCA |
| Suggested primer 2: | GCCGTATACTGACAGGTCCG |