| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.021235 |
| Chromosome: | chromosome 1 |
| Location: | 743059 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004124 | (1 of 3) 3.2.1.8 - Endo-1,4-beta-xylanase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGAATTCTGACCCAGCATTTCTAATGTAAACTTTCGGCCCATGCGACA |
| Internal bar code: | TGTAGGTAAGTCAGGTTACTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4152 |
| LEAP-Seq percent confirming: | 98.1818 |
| LEAP-Seq n confirming: | 108 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 110 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTACTCTTCTTGGCGCC |
| Suggested primer 2: | GCTCGGCATGACGTTTTACC |