| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.021236 |
| Chromosome: | chromosome 6 |
| Location: | 3846779 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278180 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGGGTGAGCGGGAGTGACATGAGTGACGGTGGTTGACGGCTGGAATG |
| Internal bar code: | GCTATCAGTGAACCGTCTAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5025 |
| LEAP-Seq percent confirming: | 91.1765 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCGTTTGCGTTTGCTGCT |
| Suggested primer 2: | CTTGCAGTTATCACGAGCGC |