| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.021251 |
| Chromosome: | chromosome 16 |
| Location: | 2376893 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g659450 | (1 of 3) 2.4.1.228 - Lactosylceramide 4-alpha-galactosyltransferase / Histo-blood group P(k) UDP-galactose | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCACCTCATCGTCCCGCAAGCGCGGGGTACCGTATCACGCACGCACC |
| Internal bar code: | GCCATAGGTAGGGGACTTAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3659 |
| LEAP-Seq percent confirming: | 99.3464 |
| LEAP-Seq n confirming: | 152 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 153 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGCTCCTCACCCCCATTG |
| Suggested primer 2: | TTGCGCAGGAACGTTGAAAG |