Insertion junction: CLIP2.021265_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.021265
Chromosome:chromosome 3
Location:6297925
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g192000 SEC13L,SEH1 COP-II coat subunit, nucleoporin; (1 of 1) K14299 - nucleoporin SEH1 (SEH1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTCTCCCACGCCGCCACCTGCGTCCAGGCGTCTGGCCCCGGCCCCGCT
Internal bar code:GTCTTTTTGCAATATTGGTTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3416
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:153
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:153

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCGTTGTCCGTGTCTTTA
Suggested primer 2:CATGTTGGAGAAGGTGGGCT