Insertion junction: CLIP2.021265_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.021265
Chromosome:chromosome 3
Location:6297928
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g192000 SEC13L,SEH1 COP-II coat subunit, nucleoporin; (1 of 1) K14299 - nucleoporin SEH1 (SEH1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCGGTCACGGGGCTGGCGTGGGCGCACCCCGAGTACGGCAGGGTGCTG
Internal bar code:TGCTACGATACGGATAGCGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:5724
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:72
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:72

Suggested primers for confirmation by PCR

Suggested primer 1:CATGTTGGAGAAGGTGGGCT
Suggested primer 2:TGGCGTTGTCCGTGTCTTTA