Insertion junction: CLIP2.021281_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.021281
Chromosome:chromosome 12
Location:7650648
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g555500 (1 of 1) 2.4.2.21 - Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase / phosphoribosyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCCATCACCACCACCACCACCAGCGCCGCCGCCGCCGGCCTGCTGCTC
Internal bar code:CTGATAGTGTACTCACAAAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3591
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:55
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAAGGGTGTGTGTGTGTG
Suggested primer 2:GTTTGGAACGTGTTGGTCGG