Insertion junction: CLIP2.021282_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.021282
Chromosome:chromosome 15
Location:347347
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g801684 (1 of 1) PF12051 - Protein of unknown function (DUF3533) (DUF3533) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACACACACACACGACGACTGCACGTTTTGCGTTTTCCGTTTCTCGTGTT
Internal bar code:CCACCGGGTATAGTGGTGAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:206
LEAP-Seq percent confirming:13.6364
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:19
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTTTGTGTTGCCTGTGCG
Suggested primer 2:CACATCTCCCAACTCCACCC