| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.021320 |
| Chromosome: | chromosome 3 |
| Location: | 8885505 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205585 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCGACCCCCCCCCCCTTCACTCTCTGTGCCAGCGGTCCGATCTGCGA |
| Internal bar code: | AATTGTTTTCTTGCGGACTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1430 |
| LEAP-Seq percent confirming: | 9.09091 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCACCTCGCAAAGACCAT |
| Suggested primer 2: | ACAAGGCAGAGGCAGATGAC |