Insertion junction: CLIP2.021335_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.021335
Chromosome:chromosome 12
Location:5856135
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g533351 CLPB5 (1 of 3) K03695 - ATP-dependent Clp protease ATP-binding subunit ClpB (clpB); ClpB chaperone, Hsp100 family intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GACACGTCATGGTGGAGCCCACGTAGCCGTACACGCACACGCACACGGCG
Internal bar code:CCGTCGTTTACTTCTTAAATGG

Confirmation - LEAP-Seq

LEAP-Seq distance:259
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGCTTTGTTCCCACTGC
Suggested primer 2:AATTCCCTCATCCCAACCCG