Insertion junction: CLIP2.021368_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.021368
Chromosome:chromosome 2
Location:310265
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g075050 PTA1 (1 of 10) IPR005828//IPR020846 - Major facilitator, sugar transporter-like // Major facilitator superfamily domain; Proton/phosphate symporter CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAGTGCACGGCGCAGATGAGGAAGAGAACAAACTGCATATGGTGGGCCA
Internal bar code:TTGACGCGAGTGAGTATTAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3478
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGTTGCACTGGGTTTCCA
Suggested primer 2:TGGGAGCTGGAGTACTACCC