| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021382 |
| Chromosome: | chromosome 1 |
| Location: | 1592449 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g008600 | (1 of 1) IPR017993//IPR022751//IPR029044 - Atrophin-1 // Alpha-mannosyltransferase // Nucleotide-diphospho-sugar transferases | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGCCCTTGCGTGGCGCAAGCGAGCGAGCGGAGGACCGCGAAGCCTGCT |
| Internal bar code: | ACAATCGGAGTTGAATAGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2209 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTTCGCAGTTTCGTGCAG |
| Suggested primer 2: | GGTACGACGGAATCCTGGAC |