Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.021393 |
Chromosome: | chromosome 6 |
Location: | 83154 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre06.g249500 | MET1,DMC7,DMT1B,DMT1 | (1 of 4) K00558 - DNA (cytosine-5)-methyltransferase 1 (DNMT1, dcm); Cytosine-C5 specific DNA methyltransferase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGGCATCCCCAGCAGCCCACCCCTTCCGCAACGTCTGGAACCTGAGTG |
Internal bar code: | ACAATAGAGATTCGATCCTGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 2212 |
LEAP-Seq percent confirming: | 92.3077 |
LEAP-Seq n confirming: | 24 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCACTGTGTCGGTGTCAGTA |
Suggested primer 2: | CGGCGCTCTGGTAAGTTGTA |