Insertion junction: CLIP2.021393_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.021393
Chromosome:chromosome 6
Location:83154
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g249500 MET1,DMC7,DMT1B,DMT1 (1 of 4) K00558 - DNA (cytosine-5)-methyltransferase 1 (DNMT1, dcm); Cytosine-C5 specific DNA methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATGGCATCCCCAGCAGCCCACCCCTTCCGCAACGTCTGGAACCTGAGTG
Internal bar code:ACAATAGAGATTCGATCCTGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2212
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GCACTGTGTCGGTGTCAGTA
Suggested primer 2:CGGCGCTCTGGTAAGTTGTA