| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021396 |
| Chromosome: | chromosome 12 |
| Location: | 6018219 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534500 | (1 of 1) PTHR20955//PTHR20955:SF1 - UNCHARACTERIZED // PROTEIN JAGUNAL HOMOLOG 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACCACCTCCCAATCGCAGGTCCACAGCCACGCAGCACACCCGCACACAC |
| Internal bar code: | TATGGTGTCGTAAAGGATTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1768 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATCTGGGCCCTTGCAAGG |
| Suggested primer 2: | GGTTGGTTGGGTAGGTAGGC |