| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.021406 |
| Chromosome: | chromosome 1 |
| Location: | 4118597 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027350 | TMG10 | (1 of 2) PTHR12029:SF11 - METHYLTRANSFERASE TARBP1-RELATED; tRNA (guanosine-2'-O)-methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACTTCACAGGTGAGCGAAACACTTTCAATTATGCAACATAATGCAGC |
| Internal bar code: | GCGACTCGGTAAATGTTGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 503 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACCTCCGAATCAGCTACC |
| Suggested primer 2: | CTGTGATTGCAGCGAGATGC |