Insertion junction: CLIP2.021407_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.021407
Chromosome:chromosome 17
Location:693123
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g700650 GFY2 (1 of 5) PTHR30178 - INNER MEMBRANE PROTEIN YAAH; Putative acetate transporter CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTAGGAGGCGAAGGCAGTGCCGCCAAAGGTGTTGCCCTTGATCAGCTGC
Internal bar code:TTGACTATCGCGATAAACTCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2144
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TGGGTATAGGGCGGGTTACA
Suggested primer 2:GGTAATCGTCTAGGCTGCCC