| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021409 |
| Chromosome: | chromosome 6 |
| Location: | 3428411 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278098 | MCCA,MCC1 | Methylcrotonoyl-CoA carboxylase alpha subunit; (1 of 2) 6.4.1.4 - Methylcrotonoyl-CoA carboxylase / Methylcrotonyl-CoA carboxylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCAAGGGGGTTTCCTCGCTGCCACGCTGATCACGTGGGCACCCGCAC |
| Internal bar code: | GCGTCGCTTCGACGCAAAAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 368 |
| LEAP-Seq percent confirming: | 25.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTAGCCTCCTCCCAAAGC |
| Suggested primer 2: | CTTGCTCACTTGCCCTCTCA |