Insertion junction: CLIP2.021414_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.021414
Chromosome:chromosome 16
Location:6505235
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g674700 AGH1 ADP-ribosyl glycohydrolase; (1 of 1) 3.2.2.24 - ADP-ribosyl-[dinitrogen reductase] hydrolase / Dinitrogenase reductase activating glycohydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGGATGACTCGGAGCTGGCTCTGGCGCTGGCGTCAGGCCTGTCTCAGG
Internal bar code:TAGACACTAGGTATGTCTCGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1966
LEAP-Seq percent confirming:93.1507
LEAP-Seq n confirming:68
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:GCGAGGAGTCATTAGTGCGA
Suggested primer 2:GGACAGACCTGCAGGAAGAC