Insertion junction: CLIP2.021443_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.021443
Chromosome:chromosome 17
Location:576037
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g699700 Hypothetical protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCCCTTGCCTCACAGGTTGGGCAGCCGGGCCGGCGCAAGTCCCGGTC
Internal bar code:ACCGCCTAAAGTAGTTGTGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:6185
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:106
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:106

Suggested primers for confirmation by PCR

Suggested primer 1:CTACACAAGGTCCGGCAACT
Suggested primer 2:CTTTTCCTTATCGCAGCGCC