Insertion junction: CLIP2.021451_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.021451
Chromosome:chromosome 10
Location:4328890
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g449750 PKS1 (1 of 1) 1.1.1.100//1.3.1.39//2.3.1.38//2.3.1.39//2.3.1.41//2.3.1.85//3.1.2.14//4.2.1.59 - 3-oxoacyl-[acyl-carrier-protein] reductase // Enoyl-[acyl-carrier-protein] reductase (NADPH, Re-specific) / Enoyl-[acyl-carrier-protein] reductase (NADPH, A-specific) // [Acyl-carrier-protein] S-acetyltransferase / Acyl-carrier-protein acetyltransferase // [Acyl-carrier-protein] S-malonyltransferase / MCAT // Beta-ketoacyl-[acyl-carrier-protein] synthase I / KAS I // Fatty-acid synthase // Oleoyl-[acyl-carrier-protein] hydrolase / S-acyl fatty acid synthase thioesterase // 3-hydroxyacyl-[acyl-carrier-protein] dehydratase / D-3-hydroxyoctanoyl-[acyl carrier protein] dehydratase; Type I polyketide synthase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGCAGCCTCCAGGCATTCACAGTCCACGACAATCCGCACCAGCACCCC
Internal bar code:CATGACTTCGAGCAGTCGAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3445
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCATCCACCTTGTCTGCG
Suggested primer 2:CAGGTGTGTGCATGTGTGTG