Insertion junction: CLIP2.021453_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.021453
Chromosome:chromosome 11
Location:631406
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467612 (1 of 1) PTHR10108//PTHR10108:SF924 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCTGGTGTGCGGCAACACGGCCTCCATGGTGGGCGAGTCCTGGCTGGC
Internal bar code:GGAGAGGATTCAGGGAGACATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1917
LEAP-Seq percent confirming:94.8718
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:ACACCTGATGGCAGCCTTAC
Suggested primer 2:CACTTGAGCACAGCCTCTCA