Insertion junction: CLIP2.021456_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.021456
Chromosome:chromosome 7
Location:2534090
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g329800 HSFL Similar to heat shock factor binding protein 1; (1 of 1) PF06825 - Heat shock factor binding protein 1 (HSBP1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCAATCTTGGCACCCATGTCGTCAATTTTACTACAAACACACGAGCAA
Internal bar code:CTGACGTTTTCGCTCATAACCA

Confirmation - LEAP-Seq

LEAP-Seq distance:4159
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:68
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:68

Suggested primers for confirmation by PCR

Suggested primer 1:CAAAGTCAAACGCAGTCGCA
Suggested primer 2:AAGGAACGGATGCTGAAGGG