| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.021462 |
| Chromosome: | chromosome 16 |
| Location: | 4704127 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g667200 | SMM47 | (1 of 2) 2.1.1.60 - Calmodulin-lysine N-methyltransferase; S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGTGTTGGCAGTGGCGGCGTCAGCAGTTGGCGCACGTGGTGGGCTGG |
| Internal bar code: | AAGATTGTACTTTATTAGTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1422 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGGTCCACGTCCACAAC |
| Suggested primer 2: | CGAAGACAGGAGCAGTGGTT |