| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.021479 |
| Chromosome: | chromosome 12 |
| Location: | 39379 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g483550 | VPE1 | (1 of 1) K01369 - legumain (LGMN); Vacuolar processing enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGAGCGGCGCCGGCGATAAATGTCCACCCCCCGGCTCGGTGGCGCGC |
| Internal bar code: | CTAGTGGGTCAGCCCGAGTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 962 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATTGGAAAGCGTGTGTGC |
| Suggested primer 2: | GTGCTGCTGAATGCGAAACA |