| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.021480 |
| Chromosome: | chromosome 10 |
| Location: | 3861336 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446100 | TRX6,TRXY,TRXY1 | Thioredoxin y, chloroplastic; (1 of 1) PTHR10438:SF280 - THIOREDOXIN Y1, CHLOROPLASTIC-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCAGTTACATGTGGGATCACTCGCCACCGCGCGTGCGCAAGCGAGTA |
| Internal bar code: | ACTGTGAGCATAATGGTGCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3858 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGGACTTCTACGCAACC |
| Suggested primer 2: | CACATGCGCTCGTGTACATG |