Insertion junction: CLIP2.021488_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.021488
Chromosome:chromosome 7
Location:4201924
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g342000 (1 of 1) PF00651//PF12847 - BTB/POZ domain (BTB) // Methyltransferase domain (Methyltransf_18) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAATGTGATCCACCTGCCCCTCGCAGTCCATTGCGCTGGAGGCTCTGGCG
Internal bar code:TACCAGGGACTATAGGCACGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:296
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTCCAACACAGCGCAAAC
Suggested primer 2:GAGGTGCGAGAGACGTCAAA