| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.021495 |
| Chromosome: | chromosome 1 |
| Location: | 7690026 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g054050 | (1 of 1) PF09834 - Predicted membrane protein (DUF2061) (DUF2061) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAGGAAATTTGTATAGATTGCGATTGCTTGAGAGGTAGAACGATGCAG |
| Internal bar code: | GGAGCACCTATAGTGCTTTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3940 |
| LEAP-Seq percent confirming: | 98.5294 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCACTTACTGACGCGTTGA |
| Suggested primer 2: | CAAGACCCTGGCCTTTTTGC |