| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.021500 |
| Chromosome: | chromosome 3 |
| Location: | 8472772 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g212305 | FAP181 | Coiled-Coil Flagellar Associated Protein 181 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAAACACCAAGGTCCGGCCCGGGACCGCCTGTGTCTCTTCCCAAGCGCT |
| Internal bar code: | GAGCAGCCAACTTGGCCATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1095 |
| LEAP-Seq percent confirming: | 95.082 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACAGCGGACTCCACTCGTA |
| Suggested primer 2: | CGTCCATGTACCACACCACA |