| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.021511 |
| Chromosome: | chromosome 3 |
| Location: | 2870333 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g162366 | (1 of 2) 1.10.3.3 - L-ascorbate oxidase / Ascorbase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGAGTGGGTGTGAATGGGGGTGGTAGAGGCGTGGGGAACCGCTGCCGT |
| Internal bar code: | TCGCTGACGTCACTTTAATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3603 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGAGGGGTTTGTGAGAG |
| Suggested primer 2: | GGCGGTGTGTGAACCTCTTA |