| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.021512 |
| Chromosome: | chromosome 10 |
| Location: | 1176320 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g425800 | ORN1 | (1 of 1) 3.1.13.3 - Oligonucleotidase; Putative organellar oligoribonuclease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGGAACCCGGGAACTCGGTGCATTTCGGGAATGGGGACCATTGTCGC |
| Internal bar code: | GCGCCAATTATGGATCTGAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2056 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCGGATTCTCTAGCTTGC |
| Suggested primer 2: | CTACATCCAGACGTCTCCGC |