| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.021526 |
| Chromosome: | chromosome 2 |
| Location: | 4228224 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104350 | PDI5 | (1 of 1) PTHR10984//PTHR10984:SF37 - ENDOPLASMIC RETICULUM-GOLGI INTERMEDIATE COMPARTMENT PROTEIN // CELL REDOX HOMEOSTASIS PROTEIN-RELATED; Protein disulfide isomerase 5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCTGAGGTCGTAGGTGAAGCGGGCCGAGGGGATGGAGTCCGACTGGT |
| Internal bar code: | AACTCTTCCGTTGTGAGGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3830 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGCAGTGTTGGGTTGAAA |
| Suggested primer 2: | GAAAACCCACAACGCATGCT |