Insertion junction: CLIP2.021529_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.021529
Chromosome:chromosome 3
Location:549979
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g145227 (1 of 1) K12199 - vacuolar protein sorting-associated protein VTA1 (VTA1, LIP5) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCATGCACAGGACCGCCTACACACCCACACCCACACCACACACGCCAGC
Internal bar code:TAAGAAGGTACGCAAATGGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:837
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAGGCACTTCCATAAGCA
Suggested primer 2:ATGGAGTCAAGCAGCTGGAC