Insertion junction: CLIP2.021550_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.021550
Chromosome:chromosome 4
Location:2877175
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g224400 (1 of 1) 3.6.3.25//3.6.3.44 - Sulfate-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTAACGCACGACGAAGGGGATTGACGGGATGGCACGGGGTGTGGCTAG
Internal bar code:GACGTGCTATACACAACATGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3608
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:168
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:168

Suggested primers for confirmation by PCR

Suggested primer 1:CAGACGCACACAAACCAAGG
Suggested primer 2:CGCAAGTACAACCTGGCCTA