Insertion junction: CLIP2.021563_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.021563
Chromosome:chromosome 12
Location:384869
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g495600 (1 of 83) IPR029044 - Nucleotide-diphospho-sugar transferases CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATCCACATTGTTGGCAGCGTGACGCTGGCGCTGCTGCGGCGCATGCCG
Internal bar code:AGGTTAGAGTAGGAGGGACAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1962
LEAP-Seq percent confirming:95.4545
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CACTGCACACACAGGAAACG
Suggested primer 2:TAACAGCTGCGAGTAGAGCG