| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.021568 |
| Chromosome: | chromosome 5 |
| Location: | 502766 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244200 | TMA1,COTH2 | spore coat protein H; (1 of 3) PF08757 - CotH protein (CotH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGCTGGTGCATCACAAACGTCAGCAGCGAGGGCGAGGGCCGGAAGGT |
| Internal bar code: | TGGATAGCTAGGAATCGACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 521 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGACCCAGACAAGGACC |
| Suggested primer 2: | TGCAACGCATACACTGTCCT |