Insertion junction: CLIP2.021574_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.021574
Chromosome:chromosome 17
Location:5047648
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g736150 POL1C,POL1-2 DNA polymerase I, probably mitochondrial; (1 of 5) PTHR10133 - DNA POLYMERASE I CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGGGCTGGCGGCGCAAGAGCTCCTGGAGCGGCTGCAGCAGCGCGAGCT
Internal bar code:AGCTGATGACTCTGTTTGGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1165
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGCCAACAGGGACCTCTG
Suggested primer 2:TGTTGGTGGATGTTCCCTCG