Insertion junction: CLIP2.021581_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.021581
Chromosome:chromosome 1
Location:4758833
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g033000 FAP334 (1 of 2) IPR000157//IPR016024 - Toll/interleukin-1 receptor homology (TIR) domain // Armadillo-type fold; Flagellar Associated Protein 334 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCATAGCTACGGCGCTGTGCGGAAGCTCATCCGCAATGTGGAGGTGCCC
Internal bar code:TTGATCCGCTTTTCGCTTCTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2372
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GTACGTGGGGTTCAGATGCA
Suggested primer 2:TTTGGGTGCAGGGTGAAGTT