| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.021593 |
| Chromosome: | chromosome 3 |
| Location: | 7934810 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g202300 | (1 of 3) IPR000104//IPR001859 - Antifreeze protein, type I // Trypanosoma cruzi ribosomal protein P2-like | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGACGGCGGTGCAGTCAAGGGCTACCAGCCACGTCGGCGGCGTGGCGCC |
| Internal bar code: | CCACTCCCAGGAGGAATGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 847 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCTGGTAGTCTACGCCC |
| Suggested primer 2: | GCTGACATGCCGTACTGGAT |