Insertion junction: CLIP2.021616_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.021616
Chromosome:chromosome 1
Location:4802864
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g033400 TIM9 Mitochondrial inner membrane translocase subunit; (1 of 1) K17777 - mitochondrial import inner membrane translocase subunit TIM9 (TIM9) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCAGCAGCCCCGCCACCAGCACCCGCACGGCGCCCGCCGTCATCGGCAC
Internal bar code:GAACACGGACGAGTGTTTAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1817
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GCGCGCTGATGATGTCATTT
Suggested primer 2:CGGGCTTAAATGGCGTGATG