Insertion junction: CLIP2.021619_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.021619
Chromosome:chromosome 11
Location:3449601
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g477400 CYG43 (1 of 1) PF00072//PF00211//PF02518 - Response regulator receiver domain (Response_reg) // Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c); Adenylate/guanylate cyclase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGATAAACCAGGTAGGCAAGGGGGAGAGAATGTAAATACCAGCTAAAT
Internal bar code:AAGGTACAAGCTTCCGAAGTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:236
LEAP-Seq percent confirming:48.7179
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:TGACTCGTGCGCAAAACAAG
Suggested primer 2:TGCCTCTTCCTCTCCTCTCC