| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.021625 |
| Chromosome: | chromosome 3 |
| Location: | 582208 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145367 | (1 of 10) PF13578 - Methyltransferase domain (Methyltransf_24) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCATGATTTGTGTTTCACCAGCCTGCGTGCGTGCGCTGACACGCATA |
| Internal bar code: | ATGAGCAAGCATCCGCTGGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5498 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 198 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 204 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGACTCATGGGTTCTTGA |
| Suggested primer 2: | ACCACAAGCGCTATCTGGAC |