Insertion junction: CLIP2.021629_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.021629
Chromosome:chromosome 3
Location:4140895
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g172800 (1 of 1) K18753 - butyrate response factor 1 (ZFP36L) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCTCCTTTGTCTCTGTCAAGGGCAATGACGGCGGTGGTGGTGGTGGC
Internal bar code:TGTTGGTCGAACGCGCCATAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:4083
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:CATCGTCCATGCCACTACCC
Suggested primer 2:CATCCCTGGACCAACCCATG