| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.021630 |
| Chromosome: | chromosome 6 |
| Location: | 3703657 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278154 | (1 of 1) PTHR31560:SF0 - UPF0652 PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGGAGCCCCGTGTCCAAACATACTCTGATCTGCATCGTGCAGACGGGT |
| Internal bar code: | AATAAGGGGCGGATGATGGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3386 |
| LEAP-Seq percent confirming: | 96.8085 |
| LEAP-Seq n confirming: | 91 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 94 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCGACCAACATCACTGAG |
| Suggested primer 2: | ATGGAATTGGCGACACGAGT |