| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.021631 |
| Chromosome: | chromosome 7 |
| Location: | 2858419 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g332250 | (1 of 9) PF00010 - Helix-loop-helix DNA-binding domain (HLH); Basic helix-loop-helix transcription factor | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGCCGGCCCGCGCCCGCCCGTTCGCATCCATGGGCGGCACCAGCTCCC |
| Internal bar code: | ACATTTGGATAATTTCTTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2952 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGACAGATGGCGACGCTAT |
| Suggested primer 2: | TGATGACGTCAGCCAGGATG |